林家豪 助理教授

研究領域 Research Interests 目前研究的方向包括：

（一）魚類鈣離子吸收及酸鹼平衡的恆定性維持
鈣離子是動物體液中重要的電解質成分，影響著動物的生長發育、能量代謝以及其他生理功能。魚類對鈣離子的吸收能力會受到水質酸鹼值變化的衝擊。在養殖環境中，魚類的呼吸代謝、排泄物及殘餌會造成水質酸化，而在室外養殖環境中，酸雨以及工業汙染物也會造成水域環境酸化。酸化環境會抑制魚類對鈣離子吸收；此外魚類還須利用耗能的調節機制來應付酸化的環境，酸化環境對生理的影響勢將影響到魚類養殖的產出。內分泌對酸鹼平衡及鈣離子恆定性的維持扮演重要角色，雖然魚類內分泌系統與哺乳動物有許多相似度，但不盡然完全相同；此外內分泌的作用機制在不同魚種間是否一致，也需多方驗證。因此，研究比較內分泌在不同養殖魚種適應外界鈣離子濃度及酸鹼值變化時所扮演的角色，不僅可以提供操作養殖魚類所需的背景知識，並可以此基礎發展相關分析指標以篩選能夠改善養殖魚類對環境變遷適應性的添加劑。
 
（二）水生動物鹽度適應機制
鹽度是水中主要的環境因子，鹽度的變化直接影響著水生動物的生理狀態。當水域環境鹽度突然改變時，會造成生物的緊迫反應。目前已證實環境鹽度緊迫會影響水產動物的存活、成長、代謝及免疫等。因此了解水生動物的滲透壓調節機制之運作具有學術上及養殖應用上重要性。
 
（三）斑馬魚實驗模式動物
斑馬魚由於具有光週期誘發產卵、胚胎透明及發育期短、飼養容易、完整基因體資料庫、基因表現操作技術便利以及相關分子細胞生物學研究技術等優勢，使得斑馬魚成為一種被廣泛使用的實驗模式動物。本實驗室除了以斑馬魚進行動物生理研究，也利用斑馬魚平台進行藥物篩選以及探討環境毒理因子對動物體健康的衝擊。


執行計畫 Funding Projects

(一) 計畫名稱：維生素D3對魚類酸鹼平衡的調控
補助單位：科技部 (國科會)
計畫編號：MOST 109-2313-B-992-003-MY3
補助期間：2020/11/01-2023/10/31
擔任工作：計畫主持人

(二) 計畫名稱：Arginase 2參與魚類排酸及離子吸收之研究
補助單位：國科會
計畫編號：NSTC 112-2313-B-992 -003 -MY3
補助期間：2023/08/01-2026/07/31
擔任工作：計畫主持人

著作 Publications (一) 國際期刊論文 International Journal Papers  (*: 通訊作者)

1. Chia-Hao Lin*, Hung-Chi Chang, Sian-Tai Liu, Huei-Jyun Hu. 2023. Vitamin D regulates ion regulation by affecting the ionocyte differentiation in zebrafish (Danio rerio) larvae. Frontiers in Marine Science, 10:1141116. (SCI, 6/113 (5.3%) in Marine and Freshwater Biology).
 
2. Chia-Hao Lin*, Sian-Tai Liu, Yu-Chun Wang, Yi-Ling Tsou, Huei-Jyun Hu. 2022.Vitamin D regulates transepithelial acid secretion in zebrafish (Danio rerio) larvae.Frontiers in Marine Science, 9:990502. (SCI, 6/113 (5.3%) in Marine and Freshwater   Biology).
 
3. Naveen Ranasinghe, Chia-Hao Lin*, Tsung-Han Lee. 2022. Cholesterol accumulation in livers of Indian medaka, Oryzias dancena, acclimated to fresh water and seawater. Frontiers in Marine Science, 9:891706. (SCI, 6/113 (5.3%) in Marine and Freshwater Biology).
 
4. Feng-Ming Chang, Chia-Hao Lin, Su-Mei Wu. 2022. Duration of Induced Metallothionein is a Critical Factor in the Cadmium-Resistance Among Goldfsh (Carassius auratus) and Common Carp (Cyprinus carpio). Bulletin of Environmental Contamination and Toxicology, 108(4):725-730. (SCI, 187/279 (67%) in Environmental Sciences).
 
5. Chia-Hao Lin, Po-Ling Yeh, Yu-Chun Wang and Tsung-Han Lee. 2021. Dynamic regulation  of ions and amino acids in adult Asian hard clams Meretrix lusoria upon hyperosmotic salinity. Frontiers in Marine Science, 8:749418. (SCI, 6/113 (5.3%) in Marine and Freshwater Biology).
 
6. Chia-Hao Lin, Po-Ling Yeh, Tsung-Han Lee. 2021. Time‐course changes in the regulation of ions and amino acids in the hard clam Meretrix lusoria upon lower salinity challenge. Journal of Experimental Zoology A, 335(7):602-613. (SCI, 19/179 (10.6%) in Zoology).
 
7. Chia-Hao Lin*, Huei-Jyun Hu, Hsin-Ju Chuang, Yi-Ling Tsou, Pung-Pung Hwang. 2021.  Cortisol and glucocorticoid receptor 2 regulate acid secretion in medaka (Oryzias latipes) larvae. Journal of Comparative Physiology B, 191(5):855-864. (SCI, 50/176 (28.4%) in Zoology).
 
8. Sakuta H, Lin CH^#, Hiyama TY, Yamaguchi K, Shigenobu S, Kobayashi K, Noda M. 2020.     SLC9A4 in the organum vasculosum of the lamina terminalis is a [Na⁺] sensor for the control of water intake. Pflugers Arch (Eur J Physiol). 472:609–624. (^#co-first author)
 
 9. Sakuta H, Lin CH, Yamada M, Kita Y, Tokuoka SM, Shimizu T, Noda M. 2020.
 Na_x-positive glial cells in the organum vasculosum laminae terminalis produce   epoxyeicosatrienoic acids to induce water intake in response to increases in [Na⁺] in body fluids. Neurosci Res. 154:45-51.
 
10. Nomura K, Hiyama TY, Sakuta H, Matsuda T, Lin CH, Kobayashi K, Kobayashi K, Kuwaki    T, Takahashi K, Matsui S, Noda M. 2019. [Na⁺] increases in body fluids sensed by central Na_x induce sympathetically mediated blood pressure elevations via H⁺-dependent activation of ASIC1a. Neuron. 101:1-16.
 
11. Lin LY, Yeh YH, Hung GY, Lin CH, Hwang PP, Horng JL. 2018. Role of calcium-sensing  receptor in mechanotransducer-channel-mediated Ca²⁺ influx in hair cells of zebrafish larvae. Front Physiol. 9:649.
 
12. Wang PJ, Yang WK, Lin CH, Hwang HH, Lee TH. 2017. FXYD8, a novel regulator of renal  Na⁺/K⁺-ATPase in the euryhaline teleost, Tetraodon nigroviridis. Front Physiol. 8:576.
 
13. Lin CH, Hu HJ, Hwang PP. 2017. Molecular physiology of the hypocalcemic action of  fibroblast growth factor 23 in zebrafish (Danio rerio). Endocrinology. 158(5):1347-1358.

 
(二) 研討會論文 Conference Papers

1.Chia-Hao Lin*, Sian-Tai Liu, Yi-Ling Tsou, and Huei-Jyun Hu. Effects of vitamin D on acid-  base regulation in zebrafish (Danio rerio). 台灣水產學會112年度學術論文發表會. 基隆, 台灣. Abstract: P. 223. 

2.Chia-Hao Lin*, Sian-Tai Liu, Yi-Ling Tsou, and Huei-Jyun Hu. 2022. Vitamin D regulates acid secretion in zebrafish (Danio rerio) larvae. 2022年中華民國魚類學會論文發表會. 台北, 台灣. Abstract: P. 12. 

3.Chia-Hao Lin*, Huei-Jyun Hu, Hsin-Ju Chuang, Yi-Ling Tsou and Pung-Pung Hwang. 2022. Cortisol regulates acid secretion in medaka (Oryzias latipes) larvae. 台灣水產學會111年度學術論文發表會. 嘉義, 台灣. Abstract: P.85 

4.Po-Ling Yeh, Tsung-Han Lee, Chia-Hao Lin*. (2021). Effects of hypoosmotic salinity on   dynamic regulation of amino acids in hard clam (Meretrix lusoria). 2021台灣海洋生物技術學會第五屆會員大會暨科技促進海洋生技產業升級研討會. 高雄, 台灣. Abstract: P.112 

5.Naveen Ranasinghe, Chia-Hao Lin, Tsung-Han Lee. 2021. Salinity and temperature effects on cholesterol accumulation through SIRT1/LXRα/SREBP1 pathway in livers of the Indian Medaka (Oryzias dancena). Experimental Biology. San Diego, USA. FASEB J. 35: Suppl 1. doi: 10.1096/fasebj.2021.35.S1.02687.    

6.Chia-Hao Lin, Pung-Pung Hwang. 2020. New insight into the role of corpuscle of Stannius in Ca²⁺ regulation of fish. 2020年中華民國魚類學會論文發表會. 屏東, 台灣. Abstract: P.3 

7.Chia-Hao Lin*. 2020. Identification of brain sensors for body-fluid osmolality control.  International Sustainable Development Conference. Pingtung, Taiwan. Abstract: P.63 

8.Chia-Hao Lin, Yao-Ming Chang, and Shih-Lei Lai. Comparative time-ordered gene coexpression network (TO-GCN) reveals novel regulators of heart regeneration. 2019台灣斑馬魚研討會 論文摘要p.18. (高雄) 

9.Chia-Hao Lin and Noda Masaharu. Identification of brain sensors for body-fluid osmolality control. 2019 東京生化学研究会第 23 回アジア地域招聘研究者研究発表会. Abstract p.11. (Tokyo, Japan) 

10.Chia-Hao Lin and Noda Masaharu. Identification of brain sensors for body-fluid osmolality control. 2018 東京生化学研究会第 22 回アジア地域招聘研究者研究発表会. Abstract p.17. (Tokyo, Japan)