病毒感染一直是魚蝦養殖產業的重要課題，例如蝦類白點症病毒及感染魚類的神經壞死病毒，藉由研究病毒的結構蛋白基因及宿主的免疫基因組成，瞭解基因功能及作用機制，可以幫助我們找出方法對抗病毒性疾病。

 

為了對抗病原感染及減少用藥等食安問題，建議養殖漁民放養篩檢過不帶病原的魚蝦種苗，但是靈敏的篩檢方法往往需要繁複的操作，設備及成本，因此影響健康種苗的推廣。本實驗室應用新穎的核酸分子檢測技術，開發可在戶外養殖現場操作的快速篩檢方法。

 

分離自維多利亞管水母的綠色螢光蛋白其研究及應用獲得2008年的諾貝爾化學獎，本實驗室以人工養殖的多種珊瑚為材料，選殖出不同顏色的螢光蛋白基因，嘗試瞭解來自珊瑚的眾多螢光蛋白其生物功能或角色分別為何？

Jiann-Horng Leu, Chi-Hang Tsai, Jyh-Ming Tsai, Chia-Hsun Yang, Chih-Yuan Hsueh, and Hsin-Yiu Chou (2019) Identification and expression analysis of 19 CC chemokine genes in orange- spotted grouper (Epinephelus coioides). Developmental and Comparative Immunology 97:1. (SCI)

Chin-I Chang, Li-Hao Chen, Yeh-Fang Hu, Chia-Che Wu, and Jyh-Ming Tsai (2017) Determining the cleavage site for the mature antimicrobial peptide of Nile tilapia b-defensin using 2D electrophoresis, western blot, and mass spectrometry analysis. Fish & Shellfish Immunology 62:41. (SCI)

Chen, L. H., S. W. Lin, K. F. Liu, C. I. Chang, J. R. Hseu, J. M. Tsai*. 2016. Comparative proteomic analysis of Litopenaeus vannamei gills after vaccination with two WSSV structural proteins. Fish & Shellfish Immunology: 49, 306-314. (SCI) *corresponding author

 

Chang, C. I., C. F. Lee, J. M. Tsai, C. C. Wu, L. H. Chen, S. C. Chen and K. J. Lin. 2014. Development of a selective and differential medium for capsulated Lactococcus garvieae. Journal of Fish Diseases 37(8): 719–728. (SCI)

 

Liu, Y. T., C. I. Chang, J. R. Hseu, K. F. Liu and J. M. Tsai*. 2013. Immune responses of prophenoloxidase and cytosolic manganese superoxide dismutase in the freshwater crayfish Cherax quadricarinatus against a virus and bacterium. Molecular Immunology 56: 72-80. (SCI) *corresponding author

 

Yang, J. Y., C. I. Chang, K. F. Liu, J. R. Hseu, L. H. Chen and J. M. Tsai*. 2012. Viral resistance and immune responses of the shrimp Litopenaeus vannamei vaccinated by two WSSV structural proteins. Immunology Letters 148: 41-48. (SCI) *corresponding author

 

Chang, C. I., P. H. Hung, C. C. Wu, T. C. Cheng, J. M. Tsai, K. J. Lin and C. Y. Lin. 2012. Simultaneous detection of multiple fish pathogens using a naked-eye readable DNA microarray. Sensors 12: 2710-2728. (SCI)

 

Chang, C. I., C. F. Lee, C. C. Wu, T. C. Cheng, J. M. Tsai and K. J. Lin. 2011. A Selective and Differential Medium for Vibrio alginolyticus. Journal of Fish Diseases 34: 227-234. (SCI)

 

Chang, C. I., C. C. Wu, T. C. Cheng, J. M. Tsai, and K. J. Lin. 2009. Multiplex nested-polymerase chain reaction for the simultaneous detection of Aeromonas Hydrophila, Edwardsiella tarda, Photobacterium damselae and Streptococcus iniae, four important fish pathogens in subtropical Asia. Aquaculture Research 40: 1182-90. (SCI)

 

Tsai, J. M., H. C. Wang, J. H. Leu, A. H. J. Wang, Y. Zhuang, P. J. Walker, G. H. Kou, and C. F. Lo. 2006. Identification of the nucleocapsid, tegument and envelope proteins of the shrimp white spot syndrome virus virion. Journal of Virology 80: 3021-9. (SCI)

 

Leu, J. H., J. M. Tsai, H. C. Wang, A. H. Wang, C. H. Wang, G. H. Kou, and C. F. Lo. 2005. The unique stacked rings in the nucleocapsid of the white spot syndrome virus virion are formed by the major structural protein VP664, the largest viral structural protein ever found. Journal of Virology 79: 140-9. (SCI)

 

Tsai, J. M.1, H. C. Wang1, J. H. Leu, H. H. Hsiao, A. H. Wang, G. H. Kou, and C. F. Lo.   2004. Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. Journal of Virology 78: 11360-70. (SCI)

1 Both authors contributed equally to this work

 

Tsai, J. M.*, L. J. Shiau, H. H. Lee, P. W. Y. Chan, C. Y. Lin. 2002. Simultaneous detection of white spot syndrome virus (WSSV) and Taura syndrome virus (TSV) by multiplex reverse transcription-polymerase chain reaction (RT-PCR) in pacific white shrimp Penaeus vannamei. Diseases of Aquatic Organisms 50: 9-12. (SCI)

*corresponding author 

 

 

陳昱安, 蔡志明, 糖果腦珊瑚的兩種螢光蛋⽩基因選殖及重組蛋⽩質表現, 107年度台灣水產學會年會, 107.1.19, 高雄.

許晉榮, 林冠宏, 蔡志明, 低鹽緊迫對點帶石斑熱休克蛋白基因表現的影響, 104年度台灣水產學會年會, 105.1.19, 屏東.

陳延典, 蔡志明, 養殖蝦類飼料添加型疫苗之研發, 104年度台灣水產學會年會, 105.1.19, 屏東.

戴寧, 蔡志明, 建立環型恆溫核酸擴增法( LAMP )快速檢測急性肝胰腺壞死 症( AHPND ), 104年度台灣水產學會年會, 105.1.19, 屏東.

張仕承,許晉榮,黃清龍,蔡志明, 點帶石斑第一型干擾素基因選殖及特性分析, 101年度台灣水產學會年會, 102/1/19-102/1/19, 基隆, pp.1.

 

陳力豪,蔡志明, 白蝦注射白點症病毒次單位疫苗其蛋白質體組成差異之研究, 100年度台灣水產學會年會, 100/12/3-100/12/3, 高雄, pp.1.

 

劉彥廷,蔡志明, 澳洲螯蝦錳型超氧歧化酵素及原酚氧化酵素基因選殖與感染蝦類白點症病毒後的酵素活性變化, 100年度台灣水產學會年會, 100/12/3-100/12/3, 高雄, pp.1.

 

楊志勇,蔡志明, 注射兩種白點症病毒結構性蛋白質誘發白蝦抗病毒及免疫反應的差異, 99年度台灣水產學會年會, 基隆。

 

Leu, J. H., Tsai, J. M., Lo C.F. 2008. White Spot Syndrome Virus. In “Encyclopedia of Virology” Third Edition. (Mahy, B.W.J. and Regenmortel, M.v. eds.) Published by Elsevier Ltd.

 

Lo, C. F., Wu, J. L., Chang, Y. S., Wang, H. C., Tsai, J. M. and Kou, G. H. 2004. Molecular characterization and pathogenicity of white spot syndrome virus. In “Current Trends in the Study of Bacterial and Viral Fish and Shrimp Diseases.” (Lin, L.K. eds.) Vol 3, Chapter 6, pp.155-187. Published by World Scientific Publishing Co. Pte. Ltd., Singapore.